Multimodal nonlinear optical polarizing microscopy of long-range molecular order in liquid crystals

We demonstrate orientation-sensitive multimodal nonlinear optical polarizing microscopy capable of probing orientational, polar, and biaxial features of mesomorphic ordering in soft matter. This technique achieves simultaneous imaging in broadband coherent anti-Stokes Raman scattering, multi-photon excitation fluorescence, and multi-harmonic generation polarizing microscopy modes and is based on the use of a single femtosecond laser and a photonic crystal fiber as sources of the probing light. We show the viability of this technique for mapping of three dimensional patterns of molecular orientations and that images obtained in different microscopy modes are consistent with each other.

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