Paper detail

Microtubule dynamic instability: the role of cracks between protofilaments

Microtubules (MTs) are cytoplasmic protein polymers that are essential for fundamental cellular processes including the maintenance of cell shape, organelle transport and formation of the mitotic spindle. Microtubule dynamic instability is critical for these processes, but it remains poorly understood, in part because the relationship between the structure of the MT tip and the growth/depolymerization transitions is enigmatic. What are the functionally significant aspects of a tip structure that is capable of promoting MT growth, and how do changes in these characteristics cause the transition to depolymerization (catastrophe)? Here we use computational models to investigate the connection between cracks (laterally unbonded regions) between protofilaments and dynamic instability. Our work indicates that it is not the depth of the cracks per se that governs MT dynamic instability. Instead it is whether the cracks terminate in GTP-rich or GDP-rich areas of the MT that governs whether a particular MT tip structure is likely to grow, shrink, or transition: the identity of the crack-terminating subunit pairs has a profound influence on the moment-by-moment behavior of the MT.