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Enhancement of Raman Light Scattering in Dye-Labeled Rat Glioma Cells by Langmuir-Blodgett CNT-Bundles Arranged on Metal-Containing Conducting Polymer Film

We have fabricated layered nanocomposite consisting of a nanoporous anodic alumina sublayer (AOA), an ultrathin metal-containing polymer Langmuir-Blodgett (LB) film coating AOA, and multi-walled carbon nanotube (MCNT) - bundles which are arranged on the LB-film. MCNTs were preliminarily chemically modified by carboxyl groups and functionalized by stearic acid. We have experimentally observed an enhancement of Raman light scattering on surface plasmons in the LB-monolayers. This enhancement is due to charge and energy transfer. We demonstrate that propidium iodide (PI) fluorescence is quenched by the MCNT-bundles. A method of two-dimensional system imaging based on the MCNT-enhanced Raman spectroscopy has been proposed. This method has been applied to visualize focal adhesion sites on membranes of living PI-labeled rat glioma cells.

preprint2015arXivOpen access
A. S. EgorovV. P. EgorovaH. V. GrushevskayaV. I. KrotN. G. KrylovaI. V. LipnevichT. I. OrekhovskayaB. G. Shulitsky
cond-mat.mes-hallcond-mat.mtrl-sciphysics.optics
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Open access8 authors3 topics
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A. S. EgorovV. P. EgorovaH. V. GrushevskayaV. I. KrotN. G. KrylovaI. V. LipnevichT. I. OrekhovskayaB. G. Shulitsky

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