Paper detail

Cellular-scale mechanism of cell crawling responding to substrate stiffness

Biological cells are able to adapt their behaviour in response to environmental cues. Durotaxis is a phenomenon in which cells adjust their migration depending on the mechanical properties of a surrounding substrate. Although durotaxis has been studied more than two decades, basic cellular-scale mechanism of how cells regulate the motility responding to substrate stiffness remains to be elucidated. We address this issue by developing a theory utilising a mechanochemical model that integrates intracellular biochemical reactions with cellular deformation and substrate adhesion. Numerical analysis reveals that the characteristic speed and diffusion constant of cells change non-monotonically with respect to substrate stiffness, indicating the emergence of an optimal stiffness for migration. In addition, by introducing a memory effect that allows feedback from cell mechanics to the intracellular chemical reactions, the persistence time increases with substrate stiffness on a substrate softer than the optimal. We further investigate theoretically the origin of the non-monotonic dependence, that is comparable to the experimental observations, in terms of cell deformation and symmetry breaking in substrate adhesion. We believe that our study provides a unifying framework to understand complex durotactic cell migration.