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Guorong Hu

Guorong Hu appears in the imported research catalog. Authorship, coauthor and topic links are available while profile ownership is still unclaimed.

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Published work

3 published item(s)

preprint2026arXiv

DeepFilters: Scattering-Aware Pupil Engineering with Learned Digital Filter Reconstruction for Extended Depth of Field Microscopy

Extended depth of field microscopy encodes axial information into a single acquisition through engineered point spread functions, but conventional and deep optics approaches are subject to degradation in scattering tissue. We introduce DeepFilters, a scattering-aware deep optics framework that jointly optimizes a parameterized pupil filter and a digital-filter-based reconstruction network through a calibrated differentiable forward model to achieve broad generalization without retraining. Incorporating empirical scattering kernels, physics-guided regularization, and a hybrid genetic-gradient initialization strategy, DeepFilters extends the PSF from 16 micron to >400 micron in clear media and enables signal recovery beyond 120 micron deep in biological tissues, validated across fixed brain slices and sea urchin embryos.

preprint2022arXiv

Deep-learning-augmented Computational Miniature Mesoscope

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of miniaturized platforms providing micron-scale resolution across centimeter-scale FOVs. To overcome this challenge, we developed Computational Miniature Mesoscope (CM$^2$) that exploits a computational imaging strategy to enable single-shot 3D high-resolution imaging across a wide FOV in a miniaturized platform. Here, we present CM$^2$ V2 that significantly advances both the hardware and computation. We complement the 3$\times$3 microlens array with a new hybrid emission filter that improves the imaging contrast by 5$\times$, and design a 3D-printed freeform collimator for the LED illuminator that improves the excitation efficiency by 3$\times$. To enable high-resolution reconstruction across the large imaging volume, we develop an accurate and efficient 3D linear shift-variant (LSV) model that characterizes the spatially varying aberrations. We then train a multi-module deep learning model, CM$^2$Net, using only the 3D-LSV simulator. We show that CM$^2$Net generalizes well to experiments and achieves accurate 3D reconstruction across a $\sim$7-mm FOV and 800-$μ$m depth, and provides $\sim$6-$μ$m lateral and $\sim$25-$μ$m axial resolution. This provides $\sim$8$\times$ better axial localization and $\sim$1400$\times$ faster speed as compared to the previous model-based algorithm. We anticipate this simple and low-cost computational miniature imaging system will be impactful to many large-scale 3D fluorescence imaging applications.