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Observation of dynamics inside an unlabeled live cell using bright-field photon microscopy: Evaluation of organelles' trajectories

This article presents an algorithm for the evaluation of organelles' movements inside of an unmodified live cell. We used a time-lapse image series obtained using wide-field bright-field photon transmission microscopy as an algorithm input. The benefit of the algorithm is the application of the Rényi information entropy, namely a variable called a point information gain, which enables to highlight the borders of the intracellular organelles and to localize the organelles' centers of mass with the precision of one pixel.

preprint2016arXivOpen access
Renata RychtarikovaDalibor Stys
Cell BehaviorSubcellular ProcessesComputer VisionQuantitative Methods
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Open access2 authors4 topics
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Citations: 0Reviews: 0Saves: 0Code: not linkedDataset: not linkedInstitutions: 0

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Renata RychtarikovaDalibor Stys

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