Abstract
Correlated electron microscopy and cathodoluminescence (CL) imaging using functionalized nanoparticles is a promising nanoscale probe of biological structure and function. Nanodiamonds (NDs) that contain CL-emitting color centers are particularly well suited for such applications. The intensity of CL emission from NDs is determined by a combination of factors, including: particle size; density of color centers; efficiency of energy deposition by electrons passing through the particle; and conversion efficiency from deposited energy to CL emission. We report experiments and numerical simulations that investigate the relative importance of each of these factors in determining CL emission intensity from NDs containing nitrogen-vacancy (NV) color centers. In particular, we find that CL can be detected from NV-doped NDs with dimensions as small as ~ 40 nm, although CL emission decreases significantly for smaller NDs.
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