Abstract
We address the need for affordable, rapid, and easy to use diagnostic technologies by coupling an innovative thermocycling system that harnesses natural convection to perform rapid DNA amplification via the polymerase chain reaction (PCR) with smartphone-based detection. Our approach offers an inherently simple design that enables PCR to be completed in 10-20 minutes. Electrical power requirements are dramatically reduced by harnessing natural convection to actuate the reaction, allowing the entire system to be operated from a standard USB connection (5 V) via solar battery packs. Instantaneous detection and analysis are enabled using an ordinary smartphone camera and dedicated app interface.
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