Paper detail

Complete Human Mitochondrial Genome Construction Using L-systems

Recently, scientists from The Craig J. Venter Institute reported construction of very long DNA molecules using a variety of experimental procedures adopting a number of working hypotheses. Finding a mathematical rule for generation of such a long sequence would revolutionize our thinking on various advanced areas of biology, viz. evolution of long DNA chains in chromosomes, reasons for existence of long stretches of non-coding regions as well as would usher automated methods for long DNA chains preparation for chromosome engineering. However, this mathematical principle must have room for editing / correcting DNA sequences locally in those areas of genomes where mutation and / or DNA polymerase has introduced errors over millions of years. In this paper, we report the basics and applications of L-system (a mathematical principle) which could answer all the aforesaid issues. At the end, we present the whole human mitochondrial genome which has been generated using this mathematical principle using PC computation power. We can claim now that we can make any stretch of DNA, be it 936 bp of olfactory receptor, with or without introns, mitochondrial DNA to 3 x 109 bp DNA sequences of the whole human genome with even a PC computation power.